By studying the lowland leopard frog (pictured) that’s found in river drainages in Arizona, UCF and Smithsonian researchers have made a breakthrough in understanding the disease that’s the leading cause of global amphibian deaths. Photo credit: Veronica Urgiles, University of Central Florida Department of Biology.

Genes Responsible for Protecting Frogs May Play Role in Death from Global Disease

By: Robert H. Wells on

For frogs dying of the invasive chytridiomycosis disease, the leading cause of amphibian deaths worldwide, the genes responsible for protecting them may actually be leading to their demise, according to a new study published today in the journal Molecular Ecology by the University of Central Florida and the Smithsonian Conservation Biology Institute (SCBI) researchers.

The lowland leopard frog, found in river drainages in Arizona, is one of a few amphibian species in which some individuals survive infection by Batrachochytrium dendrobatidis chytrid fungus (Bd) while other individuals do not—even when they live in the same local population.

In a study of lowland leopard frogs infected with Bd, the fungus that causes the disease chytridiomycosis or chytrid, researchers found that frogs that died from the disease had higher expression of major histocompatibility complex and other immune system genes than frogs that survived it.

Those genes help organisms fight off infections and foreign substances.

“This result was totally counterintuitive and the opposite of the pattern we expected to recover,” says Anna Savage, the study’s lead author, an associate professor in UCF’s Department of Biology and former postdoctoral fellow at SCBI’s Center for Conservation Genomics (CCG).

“My previous research on these immune genes showed that some variants were associated with higher survival to Batrachochytrium dendrobatidis, so I hypothesized that those genes were enabling the frogs to have a stronger immune response that would kill the fungus,” she says. “Instead, it seems like those stronger responses are linked to susceptibility, and the genes associating with survival are linked to reduced immune function.”

Anna Savage
Anna Savage, an associate professor in UCF’s Department of Biology, is the lead of author of a new study that examined the genes that are playing a role in frogs’ worldwide population decline.

Savage says acquired immune responses can be very potent, require a lot of energy from the body, and can sometimes produce toxic byproducts that harm the host and the pathogen.

“Immune responses are much more complex than just an on-off switch,” she says. “A big part of the immune system is regulating the type, timing, and dosage of a particular response, and if any of those components get dysregulated, it can have extremely negative consequences.”

She says, for instance, Batrachochytrium dendrobatidis suppresses the host immune system by killing B and T lymphocytes. “Because those are the same cells that proliferate during acquired immune responses, producing lots of those cells might just be wasting energy on something that chytrid can easily destroy,” she says.

Amphibian populations are in decline around the world, with two-thirds of the world’s 8,000 species considered to be threatened and nearly 200 species that have already gone extinct in the last two decades. In the U.S., amphibian populations overall are declining at a rate of nearly 4 percent a year, with some areas, such as the Rocky Mountains and the West Coast, facing a higher rate of decline, according to the U.S. Geological Survey.

Although the researchers studied immune gene expression in lowland leopard frogs with chytridiomycosis, the findings may be useful for studying the disease in other frog species due to genetic similarities they share, Savage says.

Lowland leopard frogs were chosen for the study because their responses to chytridiomycosis vary from one individual to the next, unlike many other frog species that are completely susceptible to the disease or are completely resistant or tolerant.

This allowed the researchers to rule out genetic variation between species and pinpoint specific differences in lowland leopard frogs’ immune genes that predicted different responses to infection.

The frogs were collected in Arizona and shipped overnight to the Smithsonian’s National Zoo in Washington, D.C., where the infection experiments were conducted. Subsequent analyses of gene expression occurred at the Smithsonian Conservation Biology Institute’s Center for Conservation Genomics. Statistical analyses of the data were performed at UCF.

Robert Fleischer, senior scientist and head of the SCBI’s CCG, co-authored the study and was Savage’s main advisor for the research when she was a postdoctoral fellow at the Smithsonian. Fleischer says the results help in understanding why some frogs survive the disease and others do not.

“If we can solve this mystery, and we have taken a big step in that direction with this study, our hope and plan is to use this information to develop resources and strategies to mitigate the disease in the more susceptible species, and to counter the worldwide tide of extinction and endangerment caused by chytrid,” he says.

The researcher says the findings also show that acquired immune responses, such as those generated by vaccination, may not always be useful in combating invasive diseases of conservation concern.

Brian Gratwicke, a conservation biologist with SCBI; Katherine Hope, an associate veterinarian with the Smithsonian’s National Zoo; and Ed Bronikowski, senior curator of the Smithsonian’s National Zoo, were study co-authors as well.

The research was funded by a Smithsonian Institution Competitive Grants Program for Science grant, the Smithsonian’s Center for Conservation Genomics, and a Smithsonian Institution Molecular Evolution Postdoctoral Fellowship.

Savage received her doctorate in ecology and evolutionary biology from Cornell University. She is a member of UCF’s Genomics and Bioinformatics Cluster and joined UCF’s Department of Biology, part of UCF’s College of Sciences, in 2015.

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